This is a test version of Biostars. For the public version, visit https://www.biostars.org.
What Gene Information Do I Need To Screen The Gene Ontology With David?

I have list of genes, and want to analyse these genes for immune related gene ontology terms with DAVID. How should I identify my selection of genes and what further information do I need to add, to prepare input for DAVID?

gene

You would normally just paste your gene list into the gene list box on the website. Perhaps you can clarify what your question is?

A list of gene symbols - to be precise - will do the trick. Gene names will not give interpretable results. Official Symbol: PRKACA. Name: protein kinase, cAMP-dependent, catalytic, alpha.

2 answers

DAVID does not deal with miRNAs as far as I know. I do not know of a tool that analyzes miRNAs with respect to GO since I do not think that miRNAs are annotated in GO.

sir, i mean that i have genes list, that list i got by miRNA target prediction...

To SEAN DAVIS, "Identification of differentially expressed miRNAs in chicken lung and trachea with avian influenza virus infection by a deep sequencing approach" BMC Genomics 2010, 11:373doi:10.1186/1471-2164-11-373"

this article contain enriched immune related GO terms of list of genes. How can it be achieved?

Normally, when clarifying your question, you would do that as a comment. It still is not clear what you have. Do YOU have a list of genes that is of interest to you, for example, from a microarray experiment where you looked for differentially-expressed genes?

sir i have target genes of miRNA. i want to do GO analysis by DAVID and want enriched immune related GO terms. so i can do it.

sir i have target genes of miRNA. i want to do GO analysis by DAVID and want enriched immune related GO terms. how can i do it. please make me understand by an example.

Go to the DAVID website, click on "Gene Functional Classification" and then paste your gene list in the text box. Then, follow the steps outlined on the site.

Log in to answer this question.