hi all,
can anyone please help me, how to convert my fasta file into fastq file.
Any other tool or open source to do this conversion?
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suhalsuhaimi •
See answers here: convert FASTA into FASTQ using linux
Note that FASTQ files carry quality scores for each base call, so unless you have QUAL files for each FASTA file, you will end up adding extra (made-up) information during the conversion process.
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Matt Shirley
Thank for the info. But then how to I get the QUAL file for my fasta sequence.
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suhalsuhaimi •
You would have to receive these with your FASTA files - for instance if your sequences are from Sanger sequencing you can usually get quality scores.
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Matt Shirley
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Hello suhalsuhaimi!
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Thank. I already to the link. And finally I can convert it. But it don't have quality score. My velvet software did not produce any result when I try to assemble this sequence?
Any other solution?
The post contains details on that too - check out Manu's answer in it.