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primer design function for perl program

Hi all,

Although there are well enough online program for primer design, such as web tool from illumina and life.

To develop several specific primers, I want to know is there any source that I can get to confirm the function of primers, such as Complementarity, dimer,Stability...?

Thanks

perl primer
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updated by Ram Ram • written by J.F.Jiang J.F.Jiang

1 answer

Have you tried the Primer3 program? BioPerl has a wrapper for it. I think BioPython also has one.

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updated by Ram Ram • written by Jean-Karim Heriche Jean-Karim Heriche

Thanks, Michael.

I used both of the modules for perl you provided before, however, even I looked into the pm files for details, they did not provide the function for the primer selection, such as Complementarity, dimer...

• 0 views • link
updated by Ram Ram • written by J.F.Jiang J.F.Jiang

Thanks for your comment.

I can use the primer3 or other newest tools for primer design.

However, I want to know if there is any perl function or other languages to define the function for primer selection.

That's what I want.

Thanks

• 0 views • link
updated by Ram Ram • written by J.F.Jiang J.F.Jiang

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