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Masking reference for RNA-seq alignments

Hi,

I have a general question about mapping RNA-seq data to the reference genome. I was wondering whether it matters if the reference genome is masked for repeats or not when mapping RNA-seq reads? if so, should it be hard mask or soft mask?

Thanks

rna-seq
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updated by Biostar Biostar • written by thjnant thjnant

1 answer

Most aligners don't care if bases are soft-masked and there's typically no point in hard-masking for aligning RNAseq datasets (though perhaps you have a non-standard dataset). If, for some reason, you need to avoid aligning to repeat regions then use a hard-masked genome.

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written by Devon Ryan Devon Ryan

Thanks for your reply. No, I have a standard RNA-seq dataset and although I have generated the masked genomes, I used the non-masked version for alignment because I thought it should not matter for RNA-seq data because they are coming from exons which I assume contains no long range repeats.

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written by thjnant thjnant

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