Hi, Does anyone know if you should remove the genes that fail independent filtering in deseq2 (I.e have NA padj values). I've read discussion around …

I want to exstract a single SNP from my .impute file (created using IMPUTE2). I want this information as a plink file with genotype on …

<p>Hi,</p> <p>When I use the option --report-secondary-alignments in TopHat, cuffdiff reports that there are fewer fragments mapping to some genes in the genes.count_tracking file.</p> <p>Without …

I am running the somatic variant caller in VarScan on several files. On average, the input .pileup files are ~50 GB each (with both tumor …

<p>Hi all, Does anyone know how to make database for compressed file fasta.gz using blast? I am using the blast2.2.29+. I saw the tutorial of …

<p>Hi,all</p> <p>Does anyone meet this question.</p> <p>Blastn Error：</p> <p>Error ：m=0, E-value = e-141，Gaps=3, However when m=8，E-value is 2e-141, Gaps=2</p> <p>Tool：Blast</p> <p>The version number：blast-2.2.26 /blastn</p> <p>Parameter：default</p> …

<p>I've installed CUDA-BLASTP and format a database with CUDA-formatdb without problem. When I try to query the database with a sequence I get the following …

<p>I've been working on editing some code on the server at my work, I've come into a problem where we need a snp quality to …

<p>My basic understanding is that "mapped" = placed on a specific location in the ref genome, whereas "aligned" = placed on a specific location with …

<p>We've sequenced two ~7-9Mbp microbial genomes using 454 which was subsequently assembled with newbler. For the first bacteria we have 8 sequence scaffolds. These scaffolds …