I want to desgin a primer for the mRNA, now I have some cell samples, it contains gDNA, mRNA(not only the target gene) More detail: …

Hello ! For creating correlation heatmap of RT-PCR data, should we take Raw Ct-values or Delta Ct-values(normalized value) or fold change values.? thank you

Is there any difference between between eukaryotic and prokaryotic primer designing? Is there any difference between conventional PCR and RT-PCR primer design? Please help me

Hello, I have a short target DNA sequence (40 nucleotides in length) in the given transcript DNA sequence. I wanted to design the PCR primers(reverse/forward) …

Hello all, I had some miRNA samples, I performed the mapping of the miRNA reads to miRBase mature file by BWA. And the raw counts …

In PCR process, you don't know the sequence of target DNA, how can you design a primer combined the end of it?

I want to design a single primer pair for a particular genus, involving its 5 species, so that while performing PCR, we involve a single …

Hello friends, I would like to design RT PCR primers for few genes which is responsible for renal transplant (Pharmacogenomics) like cyp2c8, cyp3a5, cyp3a4, abcb1, …

Hi, For alternative splicing primer design, there have been several software available. But the needs are still not be satisfied. I want to find a …

Hi all, Generally, we can use tools such as primer3 to design the available primers for PCR. However, sometimes the primer will be located in …