Question

Mirna Primer Designing

2

Entering edit mode

12.6 years ago

Adarsh Kuamr ▴ 60

Hello

How can we design primer for mature miRNA for RT-PCR quantification?

Thank you

primer mirna • 8.5k views

ADD COMMENT • link updated 16 months ago by Ram 43k • written 12.6 years ago by Adarsh Kuamr ▴ 60

score 1 · Answer 1 · 2011-10-31

1

Entering edit mode

12.5 years ago

Michael 54k

You can't! miRNA is too short to get a sufficient primer pair.

ADD COMMENT • link 12.5 years ago by Michael 54k

Ram · Answer 2 · 2015-06-28

0

Entering edit mode

8.8 years ago

Wubin Qu ▴ 170

ADD COMMENT • link updated 16 months ago by Ram 43k • written 8.8 years ago by Wubin Qu ▴ 170