TO BLAST LARGE QUERY SEQUENCE AT ONE TIME

Question

[dinesh]

Hi community, I have near 4000 query sequences that I need to BLAST in order to find homology. to do this should I need to blast my query sequence 4000 times individually one by one or we have any alternate way to blast all query sequences all at one time.(using standalone offline)

Thanks in advance

Answer 1

[Ashutosh Pandey]

http://www.ncbi.nlm.nih.gov/books/NBK1762/

http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastDocs&DOC_TYPE=Download

You can put all of your 4000 sequences in a fasta file and give it as an input.