hello
i have a list of genes all involved in the same pathway, where i can download the fasta format of them or i must download each of them individually?i mean whether possible to download fasta format of a collection of genes involved in a pathway collectively?
thank you
2 answers
You can use WebDBGET to get data from KEGG.
You mentioned that you have gene names. You need to construct two queries.
1. For a given gene name (e.g. PGM1), use bfind to get the gene entries (e.g. hsa:5236, in human) in all species
http://www.genome.jp/dbget-bin/www_bfind_sub?dbkey=genes&keywords=PGM1
It produces a list of gene entries from several species. The gene entries for human and chimp are shown below
hsa:5236 PGM1, CDG1T, GSD14; phosphoglucomutase 1 (EC:5.4.2.2); K01835 phosphoglucomutase [EC:5.4.2.2] ptr:456908 PGM1; phosphoglucomutase 1; K01835 phosphoglucomutase [EC:5.4.2.2]
2. For a list of gene entries (e.g. hsa:5236, ptr:456908), use bget to get both nucleotide and amino acid sequences in FASTA format.
http://www.genome.jp/dbget-bin/www_bget?-f+hsa:5236+ptr:456908
A relevant thread:
Is There Any Way To Retrieve Genes' Sequences In Fasta Format Using The Kegg Orthology Code?
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What kind of accessions you have ? Ensemble/Refseq etc ?
i have just the name of genes from kegg-pathway-organism
thank you