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What is my RNA-seq data's library-type?!

Hi,

I know it's a typical question but I couldn't figure it out reading forums and manual. So I have to set this library-type for TopHat but I don't know how to find what is my library-type! I just checked the accepted_hits.bam out of TopHat in IGV and Color aligned by read strand, here is an screenshot but I am not sure if it is telling anything regarding the library type! Any advise on this is appreciated.

rna-seq igv library-type
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updated by Ram Ram • written by Parham Parham

1 answer

Thanks! So if my library is single-end does it mean that the library is unstranded?!

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updated by Ram Ram • written by Parham Parham

I have the same confusion! Have you found the answer? Could you share it? Many thanks!

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written by qin.tang • qin.tang

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