I am trying to use cnvnator on lycopersicum and it always generates 0 reads.
Hello.
I am trying to use cnvnator on lycopersicum and it always generates 0 reads. I know it should be able to run on
lycopersicum because the paper mentions cnvnator can run on non-human genomes.
I have tried several lines besides these:
/path/to/CNVnator_v0.3/src/cnvnator -root 105.dedup.realign.root \
-chrom chr01 chr02 chr03 \
-tree /path/to/lycopersicum/105.dedup.realign.bam
/path/to/CNVnator_v0.3/src/cnvnator -root 105.dedup.realign.root \
-chrom 1 2 3 \
-tree /path/to/lycopersicum/105.dedup.realign.bam
/path/to/CNVnator_v0.3/src/cnvnator -root 105.dedup.realign.root \
-genome SL2.40 \
-chrom chr01 chr02 chr03 \
-tree /path/to/lycopersicum/105.dedup.realign.bam
Do you have any idea what I am doing wrong?
Kind regards,
Nikie vos
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1 answer
Does it produce any error messages? And those 3 commands you've mentioned show inconsistency in chromosome names.
Edit: You should have used SL2.40ch00 as chromosome names etc. That should fix your problem.
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please don't delete your question once it has been answered - it may be useful for others in the future. imagine if everyone deleted their questions once answered - the site would not exist
I've cleaned up your post and replaced the real path to CNVnator with
/path/to/cnvnator/