How to use R package limma to find specific gene?
Hello,I want to find whether this gene is specific or not.What I want to use is the R package limma.
For example,this gene A has two conditions. X(disease):x1,x2,x3 Y(normal):y1,y2,y3,y4,y5,y6. How can I use the limma to calculate the p-value like t-test?
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Is this microarray data? Limma should work just fine if that is the case. What problems did you have when you ran limma?
If this isn't microarray/RNAseq or similar data where you have more than one gene being measured at a time then you'll get no benefit from using limma vs. just doing a standard T-test.