How to identify used and unused reads for an assembly performed with Abyss
Hi, All
I am doing de novo assembly in ABySS and i got the result. Now I want to know how many reads are assembled and how many are not?
Can anyone help me to find out?
Thanks in advance.
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I think there is no direct option in abyss to do that. We have to map the reads on to the assembly using bowtie2 or bwa, create a sam or bam file. Using samtools flagstats, you can find the number of reads used in the assembly.
Thank you Prakki Rama