Finding the transcript direction using stranded-specific library

Hi all,

I have paired-end stranded-specific library from Illumina sequencing, which assembled (de novo assembly) using Trinity. As I used the stranded-specific library, could you please let me know how I can determine the direction of contigs (transcripts) withing the assembly?

Thanks in advance

rna-seq stranded-specific assembly

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written 9.8 years ago by seta

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