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Which algorithm should I choose when I handle the .cel when I download the .cel from Broad CMAP

Hello,when I download the .cel files from the Broad CMAP and open them, what I see is just digital gibberish.So I download the software called Gene Expression from the Aff company.

However,there are three algorithm named RMA,MAS5,PLIER WorkFlow to choose and the results are different when choosing different algorithm.

So which algorithm should I choose? And what difference are the three algorithm?

cel

1 answer

Run the RMA method.

See: http://bmbolstad.com/misc/ComputeRMAFAQ/ComputeRMAFAQ.html

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