Hi. I'm analyzing RNA-seq data. My pipeline of RNA-seq was as below. (1) Trimmomatic exclude adapter sequences and low-quality bases from my fastq files. (2) …
<p>This is kind of follow-up thread for: <a href="http://biostar.stackexchange.com/questions/14723/trim-the-low-quality-end-of-100bp-read">http://biostar.stackexchange.com/questions/14723/trim-the-low-quality-end-of-100bp-read</a></p> <p>I tried to run <code>bwa -aln -I -q 20</code> to trim off the low-quality sequences at …