Do some of you know how to get information about which position in a miRNA that is base-paired in the hairpin?
f.ex for mmu-miR-10b-5p, position 2 is not base-paired.
•
written
by
Palgrave •
Didn't you answer your question yourself?
Take the secondary structure information from miRBase. Or fold them yourself using RNAfold.
•
written
by
David Langenberger
So basically you suggest doing it manullay by looking at the stem loop base-pairing?
Well, if you have enough money to check it in-vivo, I would suggest that. :) Otherwise, I would do it in-silico, yes.
Log in to answer this question.
