Sorting out doublets in flow cytometry without height or width variables

I am working with FACS data in .fcs files that have FSC-A and SSC-A measures, but not FSC-H or SSC-H. Is there an alternative way to sort out doublets or account for them?

flowcytometry offtopic

• 157 views • link

updated 1 month ago by Arup Ghosh 402 • | written 1 month ago by UrbanHuaraches 0

This post does not fit the theme of this forum.

• 0 views • link

written 1 month ago by ATpoint 9.1K

Try posting in https://www.reddit.com/r/flowcytometry/.

Cross-post: https://www.reddit.com/r/flowcytometry/comments/1vtswau/sorting_out_doublets_without_height_or_width/

• 0 views • link

written 1 month ago by Arup Ghosh 402

0 answers

No answers yet.

More posts like this

Any ways of extracting and filtering read sequences from cram files with samtools?
written 2.6 years ago by sacryt 0

I am trying to extract read sequences from a cram file. It would be easiest with pysam but this is not working for me on …
Must an experimentally proven disease-relevant gene appear significantly differentially expressed o…
written 5.5 years ago by charlesgwellem 1

Hi. I have a bulk RNA sequencing dataset from the skin of patients with systemic sclerosis (SSC) and I wish to understand why an experimentally …
renaming flow frames in a flowSet using flowCore
written 5.6 years ago by imdoingascience 4

I'm working with flow cytometry data and I'm reading a set of FCS files into a flowSet using flowCore. By default the individual frames for …
WGCNA Soft Threshold Target for RNASeq Data
written 6.7 years ago by Tyler.Akonom 0

Hi all! I'm currently working through a WGCNA with an RNASeq data set. I've been having a hard time finding out what sort of soft …
Which read counts file from miRDeep2 should be considered for differential expression analysis?
written 8.9 years ago by bioinforesearchquestions 38

Hello everyone, I would appreciate some help with my data. I want to do Differential Expression profiling for miRNA samples. Using the miRDeep2 program and …
Flow cytometry analysis using Bioconductor
written 9.8 years ago by Nitin 17

Hello, I have set of raw .fcs files from flow cytometer, I am new to computational analyses of FCS data can anybody clarify following basic …
Determining Cluster Criteria From FlowSOM/RtSNE
written 9.9 years ago by CMosychuk 2

I'm somewhat new to bioinformatics and programming so please excuse me. I used FlowSOM to cluster my data and then produced a tSNE plot from …
Vcf Library/Parser That Lets You Add New Entries, Fields, Etc
written 13.4 years ago by DG 734

So I have been using PyVCF library in python for parsing and working with VCF files. Mostly just for filtering out variants that overlap with …
Merging Various Panel For Flow Cytometry
written 13.8 years ago by Julien Textoris 43

Hi all, I'm quite new to flow cytometry, since i have mostly perfomed transcritionnal analyses before. I discovered the flowCore, flowViz, flowXXX packages in …
How To Do Pathway Analysis For Bacteria With David Or Other Tools
written 14.0 years ago by ana.l.wang 5

I'm trying to do some pathway analysis of some of mass spec data of bacterial proteins. Is there some way to analyze a large list …