I'm somewhat new to bioinformatics and programming so please excuse me.

I used FlowSOM to cluster my data and then produced a tSNE plot from flow cytometry data using R-tSNE and ggplot2:

And so this is one FCS file with 8 fluorophores (I only used compensated fluoresence area data in my tSNE, no SSC or FSC). I used flowCore to generate a flowFrame directly from the FCS and so I havent done any gating in FlowJo. How would I determine what each cluster in the legend actually means? Or what phenotype they refer to?