I wanted to ask a quick question. I have just one vial of a prey cDNA library already transformed into the Y187 yeast strain and I need to amplify it to make a larger frozen stock for future experiments, but I am concerned about accidentally losing diversity during the process. Since if someone has experience with this, can share the lab's standard protocol for safely amplifying (multiply) a Y187 prey library? Specifically, I want to know the correct plating density, whether to use SD/-Leu selection or rich YPDA media during amplification and the best method for harvesting and freezing the final stock. If someone have a protocol or even some quick notes on this, could share it with me? Thanks a lot in advance, I really appreciate the help.