This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Test data for benchmarking a pipeline

Hello everyone, I am developing a Nextflow pipeline that processes data from raw FASTQ files through to downstream analysis for both whole genome sequencing (isolate data) and shotgun metagenomics. For publication and reproducibility, I am looking for publicly available test datasets (preferably small to moderate size) that include:

Paired-end short reads WGS isolate data

Shotgun metagenomic sequencing data

The organism is not critical, but the data should be well-documented and suitable for benchmarking a full pipeline. I would appreciate any recommendations for reliable sources (e.g., specific studies, accession numbers, or curated benchmark datasets). Many thanks in advance.

nextflow isolate pipeline metagenomics wgs

1 answer

Look in the links below for actual datasets :

Metagenomics: https://www.nist.gov/programs-projects/standards-metagenomics

WGS Bacerial isolates: https://www.nist.gov/programs-projects/microbial-genomic-measurements

Or choose your pick from MGnify: https://www.ebi.ac.uk/metagenomics

Log in to answer this question.