polyA content in RNA-Seq samples

I'm analyzing my samples and a high content of polyA was detected, is that an issue?

FastQC analysis

check rna-seq quality

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updated 6 months ago by michael.ante

• written 6 months ago by pinheirofabiano •

How long should the AAA... sequence be at the end of read, so that fastqc and multiqc consider it as poly- tail

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written 6 months ago by 1769mkc

Hi,

It depends on your library prep approach. If you are working with polyA-enriched libraries, sequencing the transcripts' 3'-end it's ok.

Please provide a bit more information on what you are working on.

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written 6 months ago by michael.ante

0 answers

No answers yet.

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