This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Transcriptome splicing analysis tools

Dear all,

I have a question regarding RNA-seq data analysis. Do you know which tool(s) within the RStudio environment can be used to examine splicing differences across all transcripts not just for a single gene?

Thank you in advance for your help!

rna-sequencing splicing

1 answer

Hi,

When I still worked with transcriptomics, I used DEXSeq to perform differential exon usage. You need aligned BAM files and the transcript annotation (see here for more details).

Log in to answer this question.