Thank you so much for you answer i.sudbery! Yes I have read it too and I don't think it's totally clear either, so I guess I might not be retard after all:) No he was not using DNA seq, only RNA seq. I now got this project in my knee:)
But if I use the variants called from my own data, doesn't that mean that the bias is allready introduced in the STAR alignReads step? So I'm running wasp with an allready biased reference? Does that sound reasonable? what do you think?