Dear experts,

It's very lucky for me to find this website as recently I encounter similar problem like Normalizing TCR data.

There are several TCR-seq datesets originated from different technologies and platforms like Adaptive Biotechnologies/Illumina/Ion Torrent PGM. Some of the data, there exists raw data format but as for immunoseq data, I can only obtain processed data as far as I'm concerned. The sequence depth is different varying from each otherso the abundace analysis seems very comparable. But I am afraid that such difference is caused by differnt sequence methods and platforms.

What can I do to normalize these datesets or how can I intergrate these datesets? Could you please give me some suggestions and instructions on how to normalize TCR-seq data? I really need these datesets!!!

Looking forward to your reply.

Thanks and best regards,
Linqy