Decision making on choosing the based on tblastn result
https://blast.ncbi.nlm.nih.gov/Blast.cgi![
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I have performed tblastn using protein accession number against a refrence genome , as shown in the image attached top hit has E-low and % identity high also low where as the latter .how to decide
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What is your aim here? Asking the right question will ensure that you get the correct answer.
Query coverage is important. Other hits may only be to domains common to the proteins.