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protease candidates for my protein of interest

Hello everyone, I have a protein of interest that is undergoing cleavage during post-translational modifications. I am wondering if there is a way that I can get a list of protease candidates that this protein can be their substrate. I also have a few candidates already but I am not sure how I can check if they recognize the cleaving site on my protein of interest. Please let me know if there is any in silico method for this. I really appreciate your help and comments.

Best wishes

cleaving-site proteases protein
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written by Fatemeh • Fatemeh

Is there anyone that can help me?

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written by Fatemeh • Fatemeh

1 answer

Have you tried PeptideCutter? https://web.expasy.org/peptide_cutter/

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written by Elisabeth Gasteiger Elisabeth Gasteiger

Thanks very much, but this only gives some bacterial enzymes that can cut my protein, unfortunately.

I was thinking maybe there are some prediction tools that I can use.

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written by Fatemeh • Fatemeh

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