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How to convert .ab1 to fastq

Good afternoon even

I actually need a help regarding How to convert ".ab1" file taken from sanger sequencing platform to its "fastq" file, with the original quality scoring for each based calls

I am doing it using chromas, SnapGene, Galaxy tools but I am not getting the correct form of fastq file could anybody please suggest me?

Or I have a doubt like whether it can be converted or not once after getting .ab1 file to fastq?

fastq
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updated by size_t • size_t • written by dr.suvarnakapale • dr.suvarnakapale

2 answers

AB1 to FASTQ

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written by Pierre Lindenbaum Pierre Lindenbaum

try this gui tool: https://technelysium.com.au/wp/chromas/

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written by size_t • size_t

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