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Gene ontology and NCBI blast analysis

I have a set of 900 FASTA sequences that were differentially expressed during a specific stimulation related to synaptic plasticity. Using Biopython, I performed a BLAST search via NCBI and shortlisted 5 key hits. Now, I want to categorize these sequences based on their functions or activities. Would Gene Ontology (GO) analysis be a suitable approach for this? As someone new to both bioinformatics and Biopython, I would appreciate guidance on how to start and which tools or databases to use. Thank you

gene-ontology protein-sequence blast
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updated by Istvan Albert Istvan Albert • written by souparnika • souparnika

1 answer

The approach that you describe is called Blast2Go

Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research

Conceptually the process is simple, but resolving all the details make it a parameter and settings sensitive.

There is even a company that provides this annotation as a service, also called Blast2Go

https://www.blast2go.com/

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written by Istvan Albert Istvan Albert

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