Error from Using FindVariableFeatures Function in Seurat Package

When I use FindVariableFeatures to process a Seurat object:

Seurat_object<- FindVariableFeatures(Seurat_object, 
                      selection.method = "vst", 
                      nfeatures = 2000)

I encounter an error with the following message:

Error in simpleLoess(y, x, w, span, degree = degree, parametric = parametric, : (converted from warning) pseudoinverse used at -2.1703

Does anyone know how to resolve this issue? Any guidance is appreciated!

findvariablefeatures seurat

• 1,992 views • link

written 1.5 years ago by Binghong •

1 answer

can you try FindVariableFeatures(Seurat_object, selection.method = "vst", nfeatures = 2000, loess.span = 0.5) increase the loess.span

• 0 views • link

updated 1.5 years ago by GenoMax

• written 1.5 years ago by Ming Tommy Tang

Thanks for your guidance, I have a try!

• 0 views • link

written 1.5 years ago by Binghong •

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