Building pangenome graphs in minigraph-cactus using vcf?
Hi everyone, in the minigraph-cactus pangenome pipeline, normally several fasta files are used. However, I am curious, is there any way to utilize cactus to build pangenome graphs using fasta+vcf?
pangenome
vcf
cactus
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Best practice to merge per sample variant calls from personalized pangenomes
written by Tobias •Hi everyone, I am aiming to identify and genotype structural variants in a cattle cohort by mapping short reads against a [Minigraph-Cactus][3] pangenome graph. To …
-
Type of Pangenome Type/program to use for fungal isolates of the same species, but have major trans…
written by Drew •Hi, I am trying to use a pangenome approach to compare 5 Fusarium proliferatum isolates that have been assembled using Nanopore/Illumina reads. However, one of …
-
Creating pangenome graphs using vcf files?
written by Uveyik •Which tools support building pangenome graphs using vcf. I know vg uses fasta+vcf. Is there anyother tools using vcf? Especially does minigraph-cactus supports vcf?
-
Rank in rGFA files
written by tsnorri •The [rGFA format](https://github.com/lh3/gfatools/blob/master/doc/rGFA.md#the-reference-gfa-rgfa-format) requires that each segment record has a rank which is specified with the `SR` tag. If the segment is on a linear …
-
Stable coordinates in gaf files?
written by Uveyik •In [gaf files][1] do we have to use rgfa reference to obtain stable coordinates? And which tools creates gaf with stable coordinates? Also, is gaf …
-
Distinguishing gfa and rgfa files
written by guntul •Hi everyone, I am confused about distinguishing gfa file and rgfa, . It seems that rgfa files also generally has `.gfa` extension. How to differentiate …
-
Key-value pairs at the end of lines of gaf/paf files
written by guntul •Hi everyone, I dont understand what the optional strings at the end lines of paf and gaf files really mean? [This link][1] and [this link][2] …
-
How can I convert a MAF of HAL (from cactus-hal2maf) to Multiple FASTA?
written by Mimi33 •(I edited some typo.) Hi, I am planning to using `GERP++` with a whole genome alignment. I want to get a multiple alignment FASTA file …
-
Extract alignment between two samples from a GFA?
written by cmdcolinHi all I am trying to learn about graph genomes, and was wondering if there was a way to "extract a pairwise alignment" between two …
-
vg rna index
written by Yumin •Hi! I'm using `vg rna` for transcriptomic analyses following the [wiki](https://github.com/vgteam/vg/wiki/Transcriptomic-analyses). I will list each of my steps and confusion below: - First, I started …
Not familiar with that tool, but it does sound like you're trying to build a variation graph instead of a reference graph.