Differentiate CLR and Hifi readings in datasets
Hi,
I would like to use minimap2 for analyzing Nanopore and PacBio data. Depending on the version, one of ax options is recommended. Ex: PacBio CLR genomic reads -ax map-pb. While many research papers detail the methodology of library preparations and kits used. It didn't specify, for example, whether PacBio reads are CLR or Hifi. How could I retrieve this kind of information?
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GenoMax
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María José •
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María José •
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I would think that
map-pbshould cover all reads from PacBio as a technology because those reads will have a certain error profile as far asminimap2goes.https://www.pacb.com/blog/hifi-difference-not-being-clr/ should help clarify.