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RNA seq analysis in R

I have gene expression data from mice, and I want to apply wald test on each dds to compare:

Day 0

  1. Control vs low
  2. Control vs high
  3. High vs low

Day 1

  1. Control vs low
  2. Control vs high
  3. High vs low

Day 3

  1. Control vs low
  2. Control vs high
  3. High vs low

Day 7

  1. Control vs low
  2. Control vs high
  3. High vs low

I already find signficant genes of each condition but the problem is that can I make pca and heatmap of each condition?

r pca heatmap

Yes? Just limit the samples to those in the comparison of interest. Not sure what the question really is.

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