How many genes is required for ORA analysis?
I did pseudobulking on my single cell data and then I compared my conditions for specific cell type. I didn't get many significant results after doing deseq. I have only 10 genes significantly changed. Can ı use them in enrichment analysis ?
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written
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carolofharvest •
1 answer
In general over-representation analysis is a brute force approach and once you have few genes the concepts don't apply.
For example a single gene even it is known to cause a phenotype will never come up via an ORA analysis.
As to how many you need - it all comes down to what kind of functions you have.
I would suggest using my tool, Genescape, I have designed it for this purpose to allow people to draw and visualize the functional content of smaller lists of genes
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