I’m relatively new to biostatistics and face some difficulties interpreting my results. I’ve read through the manual and the tutorial on the sensitivity analysis for pseudo-count addition, but I’m still a bit unclear on some of the details.
From what I understand, the sensitivity analysis tests how adding different pseudo-counts (values between 0.01 and 0.5) to zero counts in the data affects the results. The idea is to see if the p-values change significantly when different pseudo-counts are used. However, I’m not sure what to make of this in terms of reliability:
If the p-values fluctuate across the different pseudo-counts, does that mean the taxon’s results could be unreliable? Does this suggest that the results might be prone to false positives, where a taxon is incorrectly identified as differentially abundant? I would really appreciate a clearer explanation of how this sensitivity analysis works and what it means for the validity of my findings.