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Regarding to Two-color microarray data analysis (DGE)

HI dear friends

How should I do DEG analysis based on Two-color microarray data(GSE24230), and from database,I have gotten ratio such as log2(treatment/control) and log2(control/treatment) (Dye-swap experiment).

So how should I do DEG analysis based on these data

Thank you so much

differential-expression-analysis
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updated by Ram Ram • written by SHIRUI • SHIRUI

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written by Ram Ram

1 answer

Use a bioconductor package called limma for normalization, correction of Dye swap effect and then differential expression analysis. Here is the link to the package: https://www.bioconductor.org/packages/release/bioc/html/limma.html

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written by ATRX ATRX

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