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PacBio RNAseq Isoseq Pipeline question

Hello All,

I am new to PacBio RNAseq. I have a question about the pipeline - should I map the reads (using pbmm2) on the clustered BAM (i.e. has been run through isoseq cluster2).

Thank you, Evan

isoseq pipeline pacbio rnaseq

1 answer

Recommended workflows for IsoSeq are here: https://isoseq.how/getting-started.html

Aligning to the genome follows clustering step.

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