Hi, I want to construct a transcriptome assembly from a non-model organism. It has also its published genome. I want to ask the following questions: …

Hi, I have RNA seq libraries from a non-model species. Is it okay to use a different reference de novo Transcriptome assembly of the same …

Hi, I performed de novo assembly using Canu following a hybrid method. I have both Illumina (PE) and Oxford Nanopore reads. I performed reference guided …

Hi, I recently de-novo assembled a non-model fungal genome using PacBio and Illumina reads, but now we have new RNA-seq data that needs to be …

Hi all, This might be pretty inconsequential in the end but should I use a de novo or genome guided transcriptome assembly to feed into …

Hi all, I have assembled a *de novo* transcriptome with Trinity and I was wondering which is the best approach to assess the differential expression …

Dear All, I am working a plant species whose draft genome sequence is available. I have done de novo and genome-guided assembly separately, please suggest …

Hi all, As we all know, getting as much as possible the full-length contigs is desirable during the de novo transcriptome assembly of a non-model …

Hi everybody, There are different criteria to evaluate de novo transcriptome assembly quality, which all of them have their pros and cons. What are the …

Hi friends, I used Trinity assembler for de-novo assembly of a plant transcriptome based on same group protocol *"De novo transcript sequence reconstruction from RNA-Seq: …