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Affymetrix microarray

How to do one-to-one mapping between gene and probe set with Affymetrix mircoarray data? How to select the best probe value for each gene? or How to summarize multiple probes values of one gene into one representative value?

Affymetrix microarray data

gene-expression probes microarray

If you want to learn about microarray analysis, you should look into Bioconductor from R. There are a lot of packages and workflows like arrays.

1 answer

You can follow my guidance, here: How do I convert Affymetrix ID names to gene names

You will have to modify the above code depending on which Affymetrix array that you are using. It looks like you are using the U133A.

Note that you can summarise expression values across probes targeting the same gene via the limma::avereps function - How to combine expression values of multiple probes for one gene?

Kevin

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