BUSCO analysis error
I installed BUSCO v5.7.1 using Conda and ran the following command:
./bin/busco -i abc_genomic.fna --out XYZ --out_path /path_to_output_folder/ -m genome --auto-lineage &
However, I am encountering an error as follows:
busco.Exceptions.BatchFatalError: [Errno 5] Input/output error
It would be great if someone could help me in resolving this. Thank you!
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Shakunthala Natarajan •
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If you installed BUSCO using conda, you should run it simply with
buscorather than./bin/busco. A directory specified as output path must be writeable by you. What you specified (/path_to_output_folder/) does not meet that condition.I ran busco from outside the bin folder, hence I used ./bin/busco to execute it. Thank you for pointing out the folder permission. But even after changing the permissions, I was unable to resolve the error. I then found that there are two versions of busco installed, perhaps this could also be causing the error. I plan to uninstall the old version and run the analysis again. Thank you for helping out though!