hi all,
I have a question: I have several samples with quiagen quiaseq miRNA library UDI kit, sequenced on Illumina Novaseq 6000 (1x76bp).
When I check my quality fastq file, I obtained 2 adapter sequences shown in plot. (see the figure).
Why?
Can you help me?
1 answer
Many miRNA libraries work by directly attaching an adapter to the miRNA followed by making Illumina libraries from the construct so they can be sequenced. So the reads that contain the kit specific miRNA adapter should be the real miRNA. Once that adapter ends you will sequence into the Illumina library adapter present after the miRNA adapter.
From Kit page:
In an unbiased reaction, adapters are ligated sequentially to the 3’ and 5’ ends of miRNAs. Subsequently, universal cDNA synthesis with UMI assignment, cDNA cleanup, library amplification and library cleanup are performed.
Log in to answer this question.
-
How to find the adapter sequence of an RNAseq dataset?
written by Maryam •
-
Adapter removal using cutadapt and multiqc report:
written by ayeraselvan •
-
Increasing per base G content in QC sequencing files
written by robertsr •
-
In what case there are RR reads but no LL reads when detect inversions using IGV?
written by Jingfang •
-
TruSeq Illumina adapters are BLASTed with a high confidence to some genes/terms
written by e.r.zakiev •
-
Homology search of miRNA using ncbi-blast+ standalone application?
written by khq5801 •
-
Fastq with very poor per tile quality but excellent perbase quality - is this a tampered fastQ?
written by Arun •
-
GC content RNA-seq
written by UserA •
-
Per tile quality sequence fail but blue graph
written by UserA •
-
Abnormal GC content in Whole Exome Sequencing Sample after alignment
written by Leafou •
