I am working on predicting the non-coding genes, especially the ones that code for miRNAs. I am using a tool called mirMachine to predict the miRNA genes. The workflow has been properly illustrated in this article. The workflow requires a database of mature miRNA, downloaded as miRNA.fa. After the complete execution it generated two output files: 1. mature.fa.results.tbl.hairpins.fsa and 2. mature.fa.results.tbl.hairpins.tbl.out.tbl. According to the literature, the first file contains all the pre-miRNAs and the second file contains all the mature miRNAs.
I am now looking for a way to understand what these miRNAs do, their role. What should I be doing further to understand the biological role of these miRNAs. If I were to perform a BLAST, do I have to use the pre-miRNAs or mature miRNAs? Can someone help?
0 answers
No answers yet.
Log in to answer this question.
-
Which locus should be considered the true ortholog after liftOver disagreement between chrUn and ch…
written by jhonatan.henrique •
-
Alternatives to mirBase for mirDeep2 Input
written by Jack •
-
If the names of miRNAs are the same for different species, are they the same sequence?
written by Apprentice
-
How to deal with mirdeep2 output "miRBase miRNAs not detected by miRDeep2"
written by otieno43 •
-
Merging csv files with specific headers
written by windsur •
-
Detect precursor but not mature miRNA in small RNA-seq data?
written by Rey •
-
What is the purpose of having reference genome of other species during miRDeep2?
written by nivya.james2016 •
-
microRNA Annotated by Oncotator
written by haiying.kong
-
miRNA sequencing question
written by delgrodel •
-
Seed Search Of Pattern Sequence In A Given Fasta File Database
written by Varun Gupta
