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percentage of cells in each cluster- Seurat
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unbiased clustering of these top 50 genes in seurat
written by odi •Here is a code, I have now: ```r combined <- rbind(mutant1, mutant2, mutant3) as.data.frame(combined) data_genes <- data.frame(gene = rownames(combined), combined, row.names = NULL) View(data_genes) data_genes …
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seurat-creating heatmaps
written by odi •I have three tables (results from differential expression in seurat): Table 1 is for mutant 1 vs WT Table 2 is for mutant 2 vs …
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Is it possible for a cell cluster to not have cells of a certain condition?
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How to plot the heat map of a subset of cell types using plotHeatmap from scater package
written by FantasticAI •Hi I'm using plotHeatmap (library(scater)) to visualize the accuracy of cell type annotation output from SingleR. In total I got 28 labels, but as you …
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Can I tell "RaceID" to analyze only a subset of cells which correspond to known barcodes?
written by omer.shomrat •Hey everyone, I have used Seurat on an scRNA-seq dataset to find the identities (barcodes) of all the cells that belong to a specific subset …
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Downsampling one of the sample on the UMAP clustering to match the number of cells of the other gro…
written by bioinforesearchquestionsHi friends, I have two groups WT and KO. I have roughly 950 cells in WT sample and 1700 cells in KO sample. As I …
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Annovar And Protein Sequence
written by Chris •<p>I'm using annovar for mapping 1KG data onto mRNA transcripts. Now, since I'm interested in nsSNPs, I would like to know the whole protein sequence. …