Using multiple reference genomes for alignment in dorado?
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5 weeks ago
gideonav • 0

The samples I have sequenced are a mixed culture of three known species. I know you can align in-line with the dorado basecaller and upload a reference, but is there a way to use multiple reference genomes for alignment?

dorado basecall ONT alignment • 255 views
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5 weeks ago
GenoMax 141k

You can cat the references together and use as one. Just make sure the fasta headers are unique.

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