Pseudo bulking of scATAC-seq
Hello,
I have different cell clusters generated using Signac. i want to cal enhancers and super enhancers within a cluster of interest using ROSE algorithm(python ROSE_main.py -g HG37 -i enhancers.gff -r BAM -o Hezz -s 12500 -t 2500), using this code how do i come up with the bam file for each cluster? data type is scATAC
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jared.andrews07
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Manhezz •
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Manhezz •
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Use sinto to split the bam into one for each cluster by feeding in the barcodes of cells for each cluster.
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