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Is it possible to identify the strand of an RNA using UMI?

Hello to everyone who read this question! My research group did a high-throughput sRNA using the qiagen miRNA kit that uses UMIs, nothing in the kit says whether it is stranded or not, and from what I read in the literature sRNA kits are canonically strandless. My group is trying to recover the strand information from this RNA-seq. Is it possible somehow, would UMIs help with this? This image I attached is how the kit library was made. Does it have any indication of the conservation of strand information?

thank you very much for your attention.

test

high-throughput umi srna qiagen rna-seq
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updated by i.sudbery i.sudbery • written by Carolina • Carolina

You can probably use RSeQC tool. It works pretty well for RNASeq data. I have not tested it for microRNASeq data yet. However, I am expecting it should work. Please check with the infer_experiment.py function part.

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written by bk11 • bk11

1 answer

Well, from your image, it looks to me like the read with the UMI is the reverse read.

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written by i.sudbery i.sudbery

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