Error in intraspotCorrelation function (limma package): Max iterations exceeded??

Hello, community, I am currently working on analyzing microarray data using the limma package in R. Specifically, I am using the intraspotCorrelation function for separate channel analysis of two-color data. However, I have encountered an issue with the following warning messages:

Warning messages:
1: In remlscore(y, X, Z) : reml: Max iterations exceeded
2: In remlscore(y, X, Z) : reml: Max iterations exceeded

The analysis involves a design matrix (designp) and an MAList object (RG) The warning messages occur when executing the following command:

corfit <- intraspotCorrelation(RG, designp)

I would appreciate any insights or suggestions on how to resolve this issue. If you have encountered similar problems or have expertise in using the Limma package, your assistance would be highly valuable. Thank you in advance for your help! Best regards,

microarray two-color limma intraspotcorrelation

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updated 2.5 years ago by GenoMax

• written 2.5 years ago by Erfan •

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