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BigWig file in IGV

Hi,

I have generated BigWig file from BAM file by bamCoverage, but whenever I checked it in IGV I can not see the reads. Please help me.

Best

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igv
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updated by Ram Ram • written by daffodil • daffodil

1 answer

Visualizing BAM will show you reads. bigwig is a coverage graph, it will show you coverage peaks, not individual reads. That information is part of the BAM format and is lost in the bigwig summarizing operation

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written by Ram Ram

If you want to see reads open the BAM file in the same IGV window that the BigWig was made from.

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written by GenoMax GenoMax

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