Call 5mc methylation in Pacbio revio platform
Hey guys, i 'm now trying to call 5mc probability from Pacbio revio ccs.bam, but i failed. i find the hifi-kinetic signal in revio ccs.bam, but i can not use pbmm2 map the methyaltion signal to reference genome, which is different from pacbio seqII platform. Is there anyway to call the 5mc probability from pacbio revio platform
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inedraylig •
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yh •
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yh •
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Hello,
this question is rather confusing. Are you trying to predict 5mc methylations, map methylation to a reference genome, or do both? The current pipeline offered by PacBio is Jasmine:
https://github.com/PacificBiosciences/jasmine
You may want to read through their recommended pipeline and follow that, as a start.
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