This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Issues while running abundance_estimates_to_matrix.pl

Hello,
I am trying to generate a count matrix using abundance_estimates_to_matrix.pl script in Trinity, but I keep running into the same error.

/usr/lib/trinityrnaseq/util/abundance_estimates_to_matrix.pl --est_method RSEM --gene_trans_map  --name_sample_by_basedir GSNO_rep1/RSEM.isoforms.results GSNO_rep2/RSEM.isoforms.results GSNO_rep3/RSEM.isoforms.results wt_rep1/RSEM.isoforms.results wt_rep2/RSEM.isoforms.results wt_rep3/RSEM.isoforms.results
-reading file: GSNO_rep1/RSEM.isoforms.results
-reading file: GSNO_rep2/RSEM.isoforms.results
-reading file: GSNO_rep3/RSEM.isoforms.results
-reading file: wt_rep1/RSEM.isoforms.results
-reading file: wt_rep2/RSEM.isoforms.results
-reading file: wt_rep3/RSEM.isoforms.results

Outputting combined matrix.

Error, the column headings: RSEM RSEM RSEM RSEM RSEM RSEM are not unique.  Should you consider using the --name_sample_by_basedir parameter? at /usr/lib/trinityrnaseq/util/abundance_estimates_to_matrix.pl line 247.
rsem denovo rna-seq abundance-estimate

0 answers

No answers yet.

Log in to answer this question.