Hi,
I'm working on ONT data as pod5 files. Usually, the sequencing device divides the pod5 files in two output folders pod5_pass and pod5_fail. However, this time something failed on that step so I was left with all the reads in a single folder.
I am afraid I'm not very familiar with this file format so I was wondering if anyone has advice on how to split this folder by phred score (10)?
Thanks!
1 answer
Depending on what kind of nanopore device this is it may be simpler for the lab to re-do the basecalling and give you the correct results.
However, this time something failed on that step so I was left with all the reads in a single folder.
So you actually have reads in one of the "fastq_pass"/"fastq_fail" folders or no reads at all. With just POD5 files you can re-do the basecalling (using dorado caller) and then you could use chopper (LINK) to filter the reads to avg quality you need.
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